mRNA Synthesis
mRNA, saRNA (saRNA) and circular RNA (circRNA) are powerful modalities for transient protein expression and therapeutic research. Their performance depends heavily on sequence design, synthesis efficiency, and impurity control.
Crystal NAX delivers one-stop synthesis from your coding sequence to high-quality mRNA/saRNA/circRNA. Our workflow is optimized for speed, flexibility, and quality, with custom solutions for sequence optimizations, modifications, high throughput for screening, special impurity removal and specific characterizations, supporting diverse research and preclinical use cases.
Key Advantages
- Fast and streamlined synthesis workflows, sequence to mRNA as fast as 3 weeks
- Flexible production scales to support screening and in vivo studies
- High-throughput capability for parallel construct generation
- Advanced impurity control strategies for improved RNA quality
- Compatible with diverse RNA sequences and formats
Our Promise
- 100% Sequence Accuracy
- >95% On-time Delivery
- Large, Complex Sequences
Service Details
| mRNA | saRNA | circRNA | ||
|---|---|---|---|---|
|
Size (nt) |
<3000 |
3000-7000 |
7000-15000 |
<3000 |
|
Scale |
from 100ug to 10g |
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UTR |
Not required; NAX can provide UTRs for guaranteed mRNA expression |
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PolyA |
Not required; NAX can provide various polyA formats for your selection |
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Capping |
Cap1 |
Cap1 |
Cap1 |
/ |
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Codon optimization |
Optional |
Optional |
Optional |
Optional |
|
NTP Modification |
Optional |
Optional |
Optional |
Optional |
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High Throughput Option |
Yes. Minimum 10 sequences/order.
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Purity Specifications |
>90% |
>85% |
>80% |
>90% |
|
dsRNA Removal |
Optional |
Optional |
Optional |
Optional |
|
Standard QC |
ID, Content, Purity by CE.
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Additional QC |
Cap%, tail%, polyA distribution, dsRNA, endotoxin, residual protein, etc.
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sgRNA Synthesis
Single guide RNA (sgRNA) is a critical component of CRISPR-based genome editing systems, requiring high sequence fidelity and structural integrity. Enzymatic synthesis approaches offer advantages in accuracy and reproducibility, particularly for complex or longer guide constructs.
Crystal NAX provides multiple methods for sgRNA synthesis designed to support various demanding gene editing applications in research and preclinical development settings.
Key Advantages
- Enzymatic synthesis enabling high sequence fidelity
- Support for long and complex sgRNA constructs
- Consistent and reproducible RNA quality
- Flexible formats to support diverse CRISPR systems
- In vitro transcription synthesis is also available
| Length (nt) | Scale | Modifications | Synthesis Method | Purification |
|---|---|---|---|---|
|
20-105 |
1-50OD |
Standard: 2’-OMe, Phosphorothioate Option with various custom modifications |
Chemical |
Desalt |
|
20-105 |
1-50OD |
Enzymatic |
HPLC |
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|
105-230 |
1-20OD |
Enzymatic |
HPLC |
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|
>105 |
100ug-1g |
Unmodified |
IVT |
HPLC |