Linear DNA (LDNA) Solutions
Linear DNA produced using cell-free technologies offers a fast, clean, and highly adaptable alternative to plasmid-based templates. LDNA is particularly well-suited for high-throughput mRNA synthesis and screening, rapid prototyping, and applications where speed, purity, and flexibility are critical.
Crystal NAX leverages advanced cell-free DNA synthesis platforms to enable efficient LDNA production tailored for RNA synthesis workflows.
Key Advantages
- Cell-free production minimizing host-derived contaminants
- Rapid turnaround compared to plasmid-based approaches
- High-throughput capability for parallel construct generation
- Flexible sequence design without cloning constraints
- Seamless integration with mRNA and saRNA synthesis workflows
Our Promise
- 100% Sequence Accuracy
- >95% On-time Delivery
- Large, Complex Sequences
Service Details
| Gene Length (bp) | Turnaround Time (BD) | UTR | polyA |
|---|---|---|---|
|
1-1500 |
8-10 |
Not required; NAX can provide UTR for guaranteed mRNA expression |
Not required; NAX can provide UTR for guaranteed mRNA expression |
|
1500-3000 |
10-15 |
||
|
3000-5000 |
15-20 |
||
|
>5000 |
TBD |
||
|
High Throughput Option |
Minimum 10 sequences/order |
||
Deliverables
- 1ug of lyophilized linear DNA
- COA of sequencing data and LDNA purity
Plasmid DNA (pDNA) Preparation
Plasmid DNA remains a widely used and reliable DNA format for RNA production and gene delivery applications. Traditional pDNA preparation provides a familiar and robust solution for programs requiring established vector backbones or compatibility with legacy workflows.
Crystal NAX offers high-quality plasmid DNA preparation from micrograms to milligrams as a complementary service to support diverse project needs and development strategies. Complex sequences, such as extended polyA tails and ITRs can be well supported at NAX.
Crystal NAX offers high-quality plasmid DNA preparation from micrograms to milligrams as a complementary service to support diverse project needs and development strategies. Complex sequences, such as extended polyA tails and ITRs can be well supported at NAX.
Key Advantages
- Well-established and widely accepted DNA template format
- Compatible with standard RNA synthesis and downstream workflows
- Suitable for applications requiring specific vector architectures
- Reliable performance for routine and repeat-use programs
- Flexible preparation options aligned with research-scale needs
Our Promise
- 100% Sequence Accuracy
- >90% Supercoiled Purity
- Antibiotic and Animal-Free (AA-Free) available
Service Details
| Research Grade | CMC Grade | |
|---|---|---|
|
Application |
Various research purposes |
Optimized for animal studies and CMC development |
|
Scale |
>5ug |
>100ug |
|
Supercoil Purity |
/ |
>90% |
|
Endotoxin |
/ |
<5EU/mg |
|
AA-Free |
/ |
Yes |
|
Linearization |
Optional |
Optional |